Lactobacillus plantarum CRL 759-SUPERNATANT REDUCES INFLAMMATION IN AN ENDOTOXIN INDUCED UVEITIS.

LAYÚS B; GÓMEZ M; CAZORLA SI; RODRIGUEZ MV

Congreso; Reunión Anual de Sociedades de Biociencias 2020; 2020

Uveitis is a term applied to a wide range of conditions characterized by intraocular inflammation. Corticosteroids are the first line therapy for patients with uveitis, but their side effects highlight the need for new therapeutic approaches. Our previous studies demonstrated that Lactobacillus plantarum CRL 759 supernatant (LplS) was able to decrease pro-inflammatory cytokines production in LPS-stimulated ARPE-19 cells (human retinal pigment epithelium cell line). The aim of this study was to investigate the effect of LplS in a murine experimental model of uveitis induced by endotoxin.L. plantarum was cultured in DMEM medium at 37ºC 5% CO2; LplS was obtained by filtration on 0.22µm membranes. To induce uveitis, 130 µg LPS was injected subcutaneously into C57BL/6 mice. LplS was administered as drops and Prednisolone (P) was used as anti-inflammatory control. The mice were divided into six groups: LPS (LPS injection +PBS drops); LPS+LplS (LPS injection +LplS drops); LPS+P (LPS injection +prednisolone drops) and control groups treated with PBS, LplS or P drops and a PBS injection. Twenty four hours after stimulation, the mice were sacrificed. The ocular inflammation was assessed by slit lamp microscopy and clinical scores were determined. From each mouse, one eye was used for collecting aqueous humor (AqH) and the other was enucleated for histopathological evaluation. Total proteins, TNF-α and leukocyte infiltration were determined in AqH.The clinical score of mice treated with LplS was significantly lower than the LPS group (p