Probiotic lactobacillus reinforce the intestinal epithelial barrier.

MARTÍNEZ MONTEROS MJ; BALCELLS F; PERDIGON G; MALDONADO GALDEANO C; CAZORLA SI

Congreso; Reunión Anual de Sociedades de Biociencias 2020; 2020

The epithelial layer not only serves as a passive physical barrier with external environment. Intestinal epithelial cells (IEC) sense external or endogenous danger signals and mount a robust immune response to maintaining intestinal homeostasis. We previously demonstrated that lactobacillus increase Paneth cells and intestinal antimicrobial activity to keep this balance. Our aim was to further characterized the effects of probiotics on the intestinal epithelial barrier (IEB). Mice were fed with Lactobacillus casei CRL 431 (Lc431), Lactobacillus paracasei CNCM I- 1518 (Lp1518) for 7 and 5 days, respectively, or water. On day 8 mice were killed and samples were removed.Mice fed with the probiotics increased ex vivo intestinal antimicrobial activity against Staphylococcus aureus, Salmonella thyphimurium and a multi-resistant drugs Klebsiella pneumoniae (p