Systemic immune response after oral probiotics administration against Salmonella typhimurium

LEMME DUMIT JM; CAZORLA SI; PERDIGON G; MALDONADO

Capital Federal

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; 2016

Sociedad Argentina de Inmunologia

Background: Lactobacillus caseiCRL431, Lactobacillus paracasei CNCMI-1518, and their cell walls (CW431 and CW1518, respectively) improves innateimmune response and exert an immunomodulatory effect in healthy mice. The aimof the current study was to determine whether CRL431, CW431, CNCM I-1518, andCW1518 act as a potential mucosal adjuvant of S. typhimurium attenuatedby heat-shock (HSST), and would be able to protect healthy mice against alethal oral challenge with Salmonella.Methods: BALB/c mice received during 7d probiotics supplementation.Intragastrically sensitized with HSST were performed during 3 spaced days.After 3-wks one group was challenged with Salmonella(109 CFU/mL) (SG),another group received probiotics supplementation again during 7d, at the lastday was sensitized with once dose of HSST, and after 7d were challenged with Salmonella (rsSG). Control groups (CG and rsCG, respectively) received the sametreatment without the challenged with the pathogen.Translocation of microorganisms wasevaluated. The immune response was determined by in vivo and in vitro assays.Results: After probiotic supplementations, not showed increases of CFU/g oforgans compared with infection groups (p<0.05). Delayed-typehypersensitivity test  did not show differencesin SG and rsSG compared to control groups. Increased levels of IFN-g (p<0.001), and decreased levels of TNF-a (p<0.001), IL-10 (p<0.001), and IL-6(p<0.05) were found in SG comparedto CG. Serum specific IgG did not increase the titer compared to infectiongroup (p<0.05). Total and specific IgA-S not showed increas in SG compared to CG. All test groupsshowed an increased in the opsonophagocytic uptake of macrophages, compared to non-immunesera. Conclusions: Our results showed that oral probiotics administration wasnot able to induce a Th1-immune response to Salmonella.However, these findings reinforce the important effect that probiotics haveover the innate immune response and their immunomodulatory roles.