Trypanocidal and leishmanicidal activities of Flavonoids from Argentine Medicinal species

SÜLSEN V, CAZORLA S, FRANK F, REDKO F, ANESINI C, COUSSIO J, MALCHIODI E, MARTINO V, MUSCHIETTI L.

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

American Society of Tropical Medicine and Hygiene

Lugar: Estados Unidos; Año: 2007 p. 654 - 659

0002-9637

In vitro trypanocidal and leishmanicidal activities of the flavonoids hispidulin, from Ambrosia tenuifolia, and santin, from Eupatorium buniifolium, are reported. A sensitive technique, which takes advantage of the (3H)-thymidine uptake by dividing trypanosomatids, has been adjusted for the quantification of the parasiticidal effect of natural products. The IC50 values for hispidulin and santin on Trypanosoma cruzi epimastigotes were 46.7 µM and 47.4 µM. respectively. On trypomastigotes, the IC50 values were 62.3 µM for hispidulin and 42.1 µM for santin. Hispidulin was more active than santin on promastigotes of Leishmania mexicana (IC50 = 6.0 µM vs 32.5 µM). No cytotoxic activity was observed on lymphoid cells, making hispidulin and santin potential lead compounds for the development of new natural drugs. This is the first report on the trypanocidal and leishmanicidal activities of these flavonoids and on the presence of santin in E. buniifolium. 2 2 2 2 trypanocidal and leishmanicidal activities of the flavonoids hispidulin, from Ambrosia tenuifolia, and santin, from Eupatorium buniifolium, are reported. A sensitive technique, which takes advantage of the (3H)-thymidine uptake by dividing trypanosomatids, has been adjusted for the quantification of the parasiticidal effect of natural products. The IC50 values for hispidulin and santin on Trypanosoma cruzi epimastigotes were 46.7 µM and 47.4 µM. respectively. On trypomastigotes, the IC50 values were 62.3 µM for hispidulin and 42.1 µM for santin. Hispidulin was more active than santin on promastigotes of Leishmania mexicana (IC50 = 6.0 µM vs 32.5 µM). No cytotoxic activity was observed on lymphoid cells, making hispidulin and santin potential lead compounds for the development of new natural drugs. This is the first report on the trypanocidal and leishmanicidal activities of these flavonoids and on the presence of santin in E. buniifolium. 2 2