Effects of intravaginal inoculation of a potentailly probiotic lactobacillus strain in genital tract of mice

JUÁREZ TOMÁS MARÍA SILVINA; SALVA SUSANA; SANTOS VIVIANA; ALVAREZ SUSANA; NADER-MACÍAS MARÍA ELENA FÁTIMA

San Miguel de Tucumán. Tucumán. Argentina

Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

The aims of this work were to evaluate the capability of vaginal colonization and the effects on the host of potentially probiotic Lactobacillus spp. CRL (from the CERELA Culture Collection) 1263, by employing an experimental murine model. 2-months-old female BALB/c mice were estrogenized through intramuscular injection of estradiol. At day 2 post-injection, Lactobacillus spp. CRL 1263 was intra-vaginally inoculated to mice, in four doses of 107 or 108 colony forming units per dose (CFU/dose). Animals were sacrificed by cervical dislocation at days 2, 5 and 7 post-inoculation. The injection of estradiol produced an appropriate hormonal synchronization of mice, because most of animals reached estrous cycle stage at day 2 post-injection of estradiol. Only at 2 days post-inoculation, Lactobacillus spp. CRL 1263 was recovered from vaginal samples (102-103 CFU/50ul of vaginal exudates and 102-104 CFU/g of vagina). Administration of lactobacilli did not produce structural modifications in vaginal mucosa, which was determined by histological studies of vagina. T lymphoid and myeloid populations of vaginal fluid were not altered by administration of lactobacilli, which were assessed by flow cytometry analyzing the expression of CD3 and Gr-1. The results suggest that the strain evaluated would be a safe candidate for probiotic formula to prevent urogenital infections in women.