Lactobacillus rhamnosus CRL1505 nasal administration improves recovery of T-cell mediated immunity against pneumococcal infection in malnourished mice

BARBIERI NATALIA; HERRERA MATÍAS; SALVA SUSANA; VILLENA JULIO; ALVAREZ SUSANA

Beneficial Microbes

Wageningen Academic P u b l i s h e r s

Año: 2017

1876-2883

Immunobiotic lactic acid bacteria have become an interesting alternative for the prevention of respiratory infections.Previously, we demonstrated that the nasal administration of Lactobacillus rhamnosus CRL1505, during repletion ofmalnourished mice, resulted in diminished susceptibility to the challenge with the respiratory pathogen Streptococcuspneumoniae. Considering the known alterations induced by malnutrition on T lymphocytes and the importance of this cellpopulation on the protection against respiratory pathogens, we aimed to study the effect of L. rhamnosus CRL1505 nasaladministration on the recovery of T cell-mediated defences against pneumococcal infection in malnourished mice undernutritional recovery. Malnourished mice received a balanced conventional diet (BCD) for seven days or BCD for sevendays with nasal L. rhamnosus CRL1505 supplementation during last two days of the treatment. After the treatments micewere infected with S. pneumoniae. Flow cytometry studies were carried out in bone marrow, thymus, spleen and lung tostudy T cells, and Th1/Th2 cytokine profiles were determined in broncho-alveolar lavages and serum. The administrationof CRL1505 strain to malnourished mice under recovery reduced quantitative and qualitative alterations of CD4+ T cellsin the bone marrow, thymus, spleen and lung induced by malnutrition. In addition, CRL1505 treatment augmented Th2-cytokines (interleukin 10 and 4) in respiratory and systemic compartments after pneumococcal infection. These resultsshow that modulation of CD4+ T lymphocytes induced by L. rhamnosus CRL1505 has an important role in the beneficialeffect induced by this strain on the recovery of malnourished mice. These data also indicate that nasally administered L.rhamnosus CRL1505 may represent a non-invasive alternative to modulate and improve the T cell-mediated immunityagainst respiratory pathogens in immunocompromised malnourished hosts.