ESTRADIOL IN SUPERIOR MESENTERIC GANGLION MODULATES THE OVARIAN STEROIDOGENESIS THROUGH NITRIC OXIDE

VEGA OROZCO A, ; BRONZI D, ; SOSA Z; RASTRILLA AM; CASAIS M

Congreso; Sociedad de Biología de Cuyo XXXI Reunión Científica Anual; 2013

ESTRADIOL IN SUPERIOR MESENTERIC GANGLION MODULATES THE OVARIAN STEROIDOGENESIS THROUGH NITRIC OXIDE Vega Orozco A, Bronzi D, Sosa Z, Rastrilla AM, Casais M. Lab Biol Reprod (LABIR). FQByF -UNSL, IMIBIO-SL CONICET, San Luis. E-mail: asvega@unsl.edu.ar The superior mesenteric ganglion (SMG) is formed by principal neurons, which have estrogenic receptors. The objectives of this work were to study if estradiol (E2) in SMG modifies in the ovary: 1) progesterone (P4), androstenedione (A2) and E2 release; 2) 3β-HSD and 20α-HSD (P4 synthesis and degradation enzymes, respectively) activities; and 3) nitric oxide (NO) release, on diestrous II. The SMG-Ovarian Nervous Plexus (ONP)-Ovary system was incubated with E2, tamoxifeno (Txf) (non-selective estrogenic receptors antagonist) or Txf plus E2, added in ganglion compartment. SMG and ovary, connected by the ONP, were placed in different cuvettes containing Krebs Ringer solution, pH 7.4, and incubated in a metabolic bath at 37 °C. P4, E2 and A2 (by RIA), and nitrite (by Griess method), were determined at 15, 30, 60 and 120 min of incubation. ANOVA-1 and Tukey test were used. E2 in SMG inhibited ovarian P4 release (p