GANGLIONIC EFFECT OF PROGESTERONE ON LUTEAL REGRESSION AT THE END OF PREGNANCY IN RAT

GHERSA F; VALLCANERAS S; DE LA VEGA MAGALI; RASTRILLA AM, ; CASAIS M.

Congreso; Sociedad de Biología de Cuyo XXXI Reunión Científica Anual; 2013

GANGLIONIC EFFECT OF PROGESTERONE ON LUTEAL REGRESSION AT THE END OF PREGNANCY IN RAT Ghersa F, Vallcaneras S, De la Vega Magali, Rastrilla AM, Casais M. Lab. Biol. Reprod (LABIR)- FQByF ?UNSL, IMIBIO-SL CONICET, San Luis. E-mail: ssvallca@unsl.edu.ar Previously, we demonstrated that Progesterone (P4) added to the coeliac ganglion (CG), and via the superior ovarian nerve (SON), stimulates the ovarian P4 release, decreases the luteal expression of 20α-hydroxysteroid dehydrogenase (20α-HSD), P4 degradation enzyme, and FasL, a regulator factor of the way extrinsic of apoptosis. The aim of this study was to analyze the ganglionic effect of P4, through SON, on the luteal expression of 3β- hydroxysteroid dehydrogenase (3β-HSD), P4 synthesis enzyme, and Bax and Bcl-2, regulator factors of the way intrinsic of apoptosis, in pregnant rats on 21 day, using the ex vivo CG-SON-ovary system. The system was incubated in Krebs Ringer at 37ºC, keeping CG and ovary connected by the SON, in separate compartments. P4 (10-5M) was added in the ganglion compartment [(P4)g]. At the end of incubation (180 min), the luteal expression of 3β-HSD was studied by Western Blot and the expression of Bax and Bcl-2 was analysed by RT-PCR. Student´s test with a statistical significance of p