Salmonella enterica serovar Enteritidis biofilm lifestyle induces lower pathogenicity and reduced inflammatory response in the murine model compared to planktonic bacteria.

GIACOMODONATO MN; SARNACKI SH; AYA CASTAÑEDA MDR; GAROFALO A; BETANCOURT D; CERQUETTI MC; NOTO LLANA M

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2022

0325-7541

Salmonella enterica serovar Enteritidis (S. Enteritidis) is the mostfrequent serovar involved in human salmonellosis. It has been demonstrated that about80% of infections are related to biofilm formation. There is little information aboutpathogenicity of S. Enteritidis and its relationship to biofilm production. In this regard,this study was aimed to investigate the differential host response induced by S.Enteritidis biofilm and planktonic lifestyle. To this purpose, biofilm and planktonicbacteria were inoculated to BALB/c mice and epithelial cell culture. Survival studiesrevealed that biofilm is less virulent than planktonic cells. Reduced signs of intestinalinflammation and lower bacterial translocation were observed in animals inoculatedwith Salmonella biofilm compared to planktonic group. Results showed thatSalmonella biofilm displays an impaired invasiveness of no phagocytic cells andinduces a lower inflammatory response in vivo and in vitro compared to planktonicbacteria. Taken together, the outcome of Salmonella -host interaction variesdepending on the bacterial lifestyle.