Genotyping and study of pauA and sua genes of Streptococcus uberis isolates from bovine mastitis

PERRIG MS; AMBROGGIO MB; BUZZOLA FR; MARCIPAR IS; CALVINHO LF; VEAUTE C; BARBAGELATA MS

Rev. Arg. Microbiol.

Asociación Argentina de Microbiología

Lugar: Buenos Aires; Año: 2015

This study aimed to determine the clonal relationship between 137 S. uberis isolates from bovine milk with subclinical or clinical mastitis in Argentina and assess the prevalence and conservation of pauA and sua genes. This information is critical for the rational design of a vaccine for the prevention of bovine mastitis by S. uberis. The isolates were typed by random amplified of polymorphic DNA (RAPD) and by pulsed-field gel electrophoresis (PFGE). The 137 isolates exhibited 61 different PFGE types and 25 distinct RAPD profiles. Simpson?s diversity indices were calculated for PFGE (0.983) and for RAPD (0.941) showing a high discriminatory power in both techniques. Analysis of the relationship between pairs of isolates showed 92.6% concordance between both techniques indicating that any given pair of isolates distinguished by one method tended to be distinguished by the other. The prevalence of sua and pauA genes was 97.8% (134/137) and 94.9% (130/137), respectively. Nucleotide and amino acid sequences of sua and pauA genes from 20 S. uberis selected isolates, based on their PFGE and RAPD types and geographical origin, showed an identity between 95 to 100% with respect to all reference sequences registered on Genbank. These results demonstrate that, in spite of S. uberis clonal diversity, sua and pauA genes are prevalent and highly conserved.