MECHANISM OF NEONICOTINOID TOXICITY IN HUMAN TROPHOBLAST

GOMEZ, DIEGO SEBASTIAN; BUSTOS, PAMELA SOLEDAD; GUIÑAZÚ, NATALIA

Mar del Plata

Congreso; Reunión Conjunta SAIC SAI SAFIS 2018; 2018

Sociedad Argentina de Investigación Clínica

Neonicotinoid (NEO) insecticides are a relatively new class of pesticidesdeveloped to replace other insecticides. NEO were thoughtto have limited toxicity to mammals, since their higher specificitytoward insects. Nevertheless NEO toxicity in non-target organismsis not widely studied. We have demonstrated that the NEO acetamiprid(Ace) and its commercial formulation (CFace) are cytotoxic tohuman trophoblast cell lines, and trigger reactive oxygen speciesproduction. Moreover, CFace is more toxic to trophoplasts than Ace.The aim of this study was to investigate the toxicity mechanisms ofAce and CFace in a human trophoblast cell line.Human trophoblst HTR-8/SVneo cells were exposed to Ace orCFace (0.1-100 μM) for 4 and 24 h. Antioxidant enzyme activities(catalase, superoxide dismutase and glutathione s-transferase), aswell as protein oxidation ?PO? (advanced oxidation protein productsmethod) and genotoxic damage ?GD? (comet alkaline assay) weredetermined under these conditions.Exposure to Ace and CFace modulated antioxidant enzymes:Ace reduced activities at high concentrations (10-100 μM) at 24 h(p