SURFACE MODIFICATION OF METALLIC GOLD NANOPARTICLES WITH CYSTEINE DECREASES CELL TOXICITY

LOPEZ VENDITTI, ELIANA; BUSTOS, PAMELA SOLEDAD; GOMEZ, DIEGO SEBASTIAN; RODRIGUEZ, PIUQUE MIGUEL; GARCÍA MARTÍNEZ, JOAQUIN; PÁEZ, PAULINA LAURA; GUIÑAZÚ, NATALIA

Mar del Plata

Congreso; Reunión Conjunta SAIC SAI SAFIS 2018; 2018

Sociedad Argentina de Investigación Clínica

Metal nanoparticles -NP- (10-100 nm) are applied in pharmaceuticalproducts, and the modification with anti-oxidants can improve theirproperties. The aim of the present work was to investigate differencesin the toxicity of metallic gold NP (AuNP) and those modified withcysteine (AuCysNP), on a human cell line.The chemical synthesis of AuNP and AuCysNP was carried out using CTAB (ligand) and sodium borohydride (NaBH4) as a reducingagent: 0.01M AuHCl4; CTAB 0.01M; 10mM NaBH4 and 0.025mMCysteine. The NPs were characterized by UV/VIS spectroscopy andtransmission electronic microscopy (TEM). The HTR-8 / SVneo cellline was incubated for 6 and 24 h at different NPs concentrations(0.01 - 0.5 mM). Cell viability was evaluated by MTT, the productionof reactive oxygen species (ROS) by NBT, the levels of nitric oxideby Griess, and the activity of antioxidant defense enzyme, superoxidedismutase (SOD) by riboflavin-NBT method.Exposure for 6 h to AuNP significantly decreased cell viability at 0.2and 0.5 mM whereas AuCysNP diminished cell viability at 0.5 mM.After 24 h incubation, both NPs significantly modified cell viabilityat different extent at 0.2 and 0.5 mM, AuNP decreased cell viabilitya 29 and 68%, while AuCysNP a 19 and 49%, respectively. ROSlevels were investigated after 24 h incubation, ROS increased afterAuNP (0.1-0.5 mM) incubation, while AuCysNP only increasedROS at 0.5 mM. No changes in nitric oxide levels were observedin the conditions tested. SOD activity was determined after 24 hincubation, an increase in enzyme activity was determined at allthe concentrations tested for AuNP and in 0.1; 0.2 and 0.5 mM forAuCysNP, compared to controls.These results suggest that the AuNP modification with the aminoacidcysteine decreases the cell toxicity of AuNP and it may favors anantioxidant response.