Biologically synthesized silver nanoparticles, mediated by Bothriochloa laguroides, inhibit biofilm formation and eradicate mature biofilm of Yersinia enterocolitica and Staphylococcus aureus.

TORANZO, ARACELI; BUSTOS, PAMELA SOLEDAD; ORTEGA, MARÍA GABRIELA; PÁEZ, PAULINA LAURA; LUCERO-ESTRADA, CECILIA

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2021

1364-5072

Aims: To phytosynthesize silver nanoparticles (AgNPs) and determine their antibacterial andantibiofilm capacity against gram-positive and negative bacterial strains.Methods and Results: AgNPs were synthesized using Bothriochloa laguroides aqueous extract asreducing and stabilizing agent. After characterization, a phytochemical screening to the extract andthe AgNPs was performed. Antibacterial activity, inhibition and eradication of biofilms againstStaphylococcus aureus and Yersinia enterocolitica strains were tested. Spherical AgNPs with anaverage size of 8 nm were obtained. Tannins, flavonoids, carbohydrates, proanthocyanidins,anthocyanins, and saponins were identified in aqueous extract; meanwhile, only carbohydrates wereidentified in AgNPs. The MIC and MBC were determined at pmol l-1 levels for all tested strains.Furthermore, AgNPs inhibited more than 90% of biofilms formation and eradicated more than 80% ofmature biofilms at concentrations higher than MIC.Conclusions: The AgNPs obtained in this study inhibited planktonic and sessile growth, anderadicated mature biofilms of pathogenic bacterial strains at very low concentrations.Significance and Impact of Study: The current study showed the promising potential of AgNPs asantibiofilm agents opening the way for the future development of a new class of antibacterialproducts.