Effect of poly (l-lactic acid) scaffolds seeded with aligned diaphragmatic myoblasts overexpressing connexin-43 on infarct size and ventricular function in sheep with acute coronary occlusion

C.S. GIMÉNEZ; F.D. OLEA; P. LOCATELLI; R.A. DEWEY; G.A. ABRAHAM; F. MONTINI BALLARIN; M.R. BAUZA; A. HNATIUK; A. DE LORENZI; A.N. SEPÚLVEDA; M. EMBON; L. CUNIBERTI; A. CROTTOGINI

Artificial Cells, Nanomedicine and Biotechnology

TAYLOR & FRANCIS INC

Lugar: London; Año: 2018 p. 1 - 8

2169-1401

Diaphragmatic myoblasts (DM) are stem cells of the diaphragm, a muscle displaying highresistance to stress and exhaustion. We hypothesized that DM modified to overexpressconnexin-43 (cx43), seeded on aligned poly (l-lactic acid) (PLLA) sheets would decreaseinfarct size and improve ventricular function in sheep with acute myocardial infarction(AMI). Sheep with AMI received PLLA sheets without DM (PLLA group), sheets withDM (PLLA-DM group), sheets with DM overexpressing cx43 (PLLA-DMcx43) or notreatment (control group, n=6 per group). Infarct size (cardiac magnetic resonance)decreased ~25% in PLLA-DMcx43 [from 8.2±0.6 ml (day 2) to 6.5±0.7 ml (day 45),p