NRPS in the Trichoderma koningiopsis POS7 isolate: detection and annotation

CASTRILLO M. L.; BICH G. A.; SOAREZ J. N.; VILLALBA L. L.; SAPARRAT M.C.N.; ZAPATA, PEDRO

Congreso; 2nd Latin American Congress of Women in Bioinformatics and Data Science:; 2021

Latin American Congress of Women in Bioinformatics and Data Science:

One of the mechanisms used by Trichoderma to control invasive pathogens is theproduction and release of secondary metabolites (SMs) and enzymes. In fungi, thegenes responsible for the synthesis of the SMs are often arranged in clusters on thegenome, referred to as biosynthetic gene clusters (BGCs). Non-ribosomal peptidesynthetases (NRPSs) catalyze the synthesis of a group of SMs, and consist of a series ofmodules that act as an assembly line. In this work, the presence of BGCs that showgene prediction for the NRPS in T. koningiopsis POS7 was evaluated. Our grouppreviously sequenced the genome of the T. koningiopsis POS7 isolate and assembled itinto 248 scaffolds using SPAdes software. From these 248 scaffolds, the BGCs codingfor the NRPSs were searched and identified, using antiSMASH software. In order tocorroborate the identity of the BGCs obtained, they were compared against a databasefor amino acid and nucleotide sequences, using the BLASTp and BLASTn platforms ofthe NCBI database. Only those BGCs with a complete module necessary to activateand contribute to a single unit to the synthesis of final assembled product werestudied. Although in the T. koningiopsis POS7 genome 7 BGCs were identified, only aBGC presented gene prediction for the NRPS. It was located in NODE_62 scaffoldbetween positions 26,611-51,345 bp with a predicted sequence of 7,277 aa. A corepeptide biosynthetic gene was detected, which is composed of 5 adenylation domains,7 peptidyl carrier domains, 5 condensation domains, and 1 reductase terminal domain.Several accessory genes were also detected: 1 additional biosynthetic gene and 4 othergenes with incongruous functions for the formation of SMs. These results couldfacilitate the construction of metabolic engineering strategies to increase the yield oftarget MSs in Trichoderma species, such as ones catalyzed by NRPS.