DE NOVO AND BY HOMOLOGY PREDICTION OF TRNA GENES OF THE MITOCHONDRION OF THE MYCOPARASITIC FUNGUS TRICHODERMA KONINGIOPSIS

CASTRILLO M. L.; BICH G. A.; ZAPATA P. D.; SAPARRAT M.C.N.; VILLALBA L. L.

Congreso; 1st Latin American Congress of Women in Bioinformatics & Data Science LA.; 2020

Latin American Congress of Women in Bioinformatics & Data Science LA.

Trichoderma species are common soil-borne fungi. In integrated pest management, many species of this genus are economically important, in part because of their mycoparasitic ability, which makes them suitable for application as biocontrol agents against soil-borne plant-pathogenic fungi. However, little is known about the genome of many species their species. T. koningiopsis genome was recently reported but the information about the genes located in their mitochondrion is still scarce. Mitochondria are organelles necessary for the life of eukaryotic cells, and mitochondrial gene rearrangements and the secondary structure of tRNAs are also widely used for phylogenetic studies in eukaryotes. The aim of this work was to predict the tRNA genes located in the mitochondrion of the mycoparasitic fungus T. koningiopsis. Complete mitochondrial sequences of Trichoderma were retrieved from National Center for Biotechnology Information (NCBI) database. First, examination of the presence of tRNA-like sequence was performed visually in the search by homology of tRNA genes with other mitogenomes reported of Trichoderma genus. The de novo prediction was performed by the tRNAScan online software. BLAST online service of the NCBI was used for the analysis of the tRNA genes sequence predicted from the mitochondrion of T. koningiopsis. The complete sequence of the mitochondrion of T. koningiopsis was 27,688 bp, with a 27.6% GC content, a circular shape, and the sequence analysis revealed that it contained 26 tRNA genes (7% of the total sequence length). All of the detected tRNA genes were located on the sense strand. Some transfer genes were duplicated (trnR, trnL, and trnF) and trnM triplicated. It was observed a high correlation between de novo and by homology prediction. The information obtained let us continue understanding a native biocontrol fungus, and further studies are needed to predict other genes in the mitogenome.