THE GENOME OF TRICHODERMA KONINGIOPSIS CARRIES 19 GENES ENCODING ENZYMES OF THE CELLULOLYTIC COMPLEX

CASTRILLO M. L.; BICH G. A.; ZAPATA P. D.; SAPARRAT M.C.N.; VILLALBA L. L.

Congreso; 1st Latin American Congress of Women in Bioinformatics & Data Science LA; 2020

Latin American Congress of Women in Bioinformatics & Data Science LA

Cellulose is the most abundant polysaccharide in nature. Three types of cellulase are required for cellulose hydrolysis to glucose: endoglucanases (EGs), β-glucosidases (BGLs) and cellobiohydrolases (CBHs). The Trichoderma koningiopsis POS7 fungal isolate secretes high levels of cellulolytic-active enzymes and is therefore a promising strain for use in biotechnological applications. However, the T. koningiopsis genes involved in cellulose hydrolysis has not been well explored at the genetic level. The present work analyses the genome of this fungus for the study of genes related to cellulase enzymes. Genomic DNA library construction and draft genome sequencing were performed by Macrogen using the Illumina MiSeq system. These sequences were assembled de novo using the SPAdes software. To predict genes we used two ab initio gene predictor, AUGUSTUS and FgenesH. A local database was made with the nucleotide and amino acid sequences of genes that code for cellulase enzymes (EGs, BGLs and CBHs) of other species of Trichoderma and related organisms and were compared with the annotation files generated by AUGUSTUS and FgenesH. These sequences were blasted in the NCBI database using the BLASTn and BLASTp tools in order to determine identity (ie, regions containing sequences of the genes encoding cellulases). In addition, Integrative Genomics Viewer and Geneious software were used to determine the complete structural regions of each gene of interest. The cellulase enzymes genes in the genome of T. koningiopsis POS7 were scattered in 15 assembled scaffolds. We predict and annotate 19 cellulase enzymes genes: seven EGs, ten BGLs and two CBHs. The cellulase genes were located mainly in the minus strand except for four genes encoding BGLs enzymes. The genes encoding CBHs enzymes were located in the same scaffold of two EGs enzymes genes.