Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol

DANIEL MATÍAS G. REGIART; MARCO A. SEIA; GERMÁN A. MESSINA; FRANCO A. BERTOLINO; JULIO RABA

MIKROCHIMICA ACTA

SPRINGER WIEN

Lugar: Viena; Año: 2014 vol. 182 p. 531 - 538

0026-3672

We describe an electrochemical immunosensor for the determination of the growth promoter α-zearalanol in bovine serum. The sensing scheme is based on a nanocomposite consisting of gold nanoparticles electrodeposited on multi-walled carbon nanotubes that were modified with poly(vinylpyridine) through in-situ polymerization. The electrodeposition of the gold nanoparticles enlarges the surface available for immobilization of antibodies against α-zearalanol. The nanocomposite film was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, and cyclic voltammetry. The calibration plot has a linear response in the concentrations range from 0.05 to 50 ng mL−1, and the detection limit is 16 pgmL−1. The time required for analysis is 12 min only which compares quite favorably with the time (90 min) required by the conventional ELISA. The method exhibits good selectivity, stability and reproducibility for detecting α-zearalanol in the livestock production.