CONICET | Buscador de Institutos y Recursos Humanos

Background: Genome annotation techniques and methodologies used in the assessment of fungalgenomes play a crucial role in understanding the genetic background and functionalities of theseorganisms. There are various approaches to this complex process, and it is important to evaluate the quality and reliability of the annotation performed on fungal genomes. In this context, Pleurotuspulmonarius LBM 105, a fungus isolated from Misiones (Argentina), has demonstrated qualities suitable for environmental biotechnology applications. Therefore, progress in annotating its genome represents a significant advancement in both scientific and technological understanding.Results: In this study, we examined various annotation methods applied to the de novo assembly of P. pulmonarius LBM 105 in order to evaluate their accuracy and completeness. We compared a reference annotation generated with CLC Genomic Workbench to classic ab initio annotation using the Augustus software and fungal optimized annotation software called Funnanotate. Additionally, we incorporated two different masking approaches for the ab initio annotations - one using RepeatModeler to generate repeat sequences from the genome, and another utilizing the Dfam repeat database. The most complete annotation was achieved by the reference annotation yielding a total of 13560 genes, 79283 CDS and 12694 mRNAs. However, using Augustus we achieved similar results with 13331 genes, 57933 CDS and 13331 mRNAConclusions: This level of completeness suggests that the annotation pipeline used in this studysuccessfully captured a significant portion of the fungal genome, highlighting the importance of using a high-quality reference for the genomic studies. However, the ab initio annotation results showed promising results, allowing to better capture the strain-specific characteristics of the genome

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