Decoloración y detoxificación con Stereum hirsutum. Un basidiomicete de la pudrición blanca de la madera

N. MOUSO; L. LEVIN; D.L. SALARIATO; V.L. PAPINUTTI; F. FORCHIASSIN

Segundo libro SETAC América Latina, Salud ambiental y humana. Una versión Holística

Sociedad de Toxicología y Química Ambiental (SETAC) Press

Año: 2006; p. 153 - 155

White rot fungi produce extracellular ligninolytic enzymes with a low specificity that degrade lignin as well as many phenolic and non-phenolic compounds, among them PAHs, PCBs and dyes. In this work we evaluate the ability of Stereum hirsutum to decolorize and detoxify different dyes under solid state fermentation (SSF) conditions. Three different substrates were assayed: wheat bran, soya bran and a mixture (1:1) of both. Cultures (substrate colonized by the fungus) were assayed for their ability to decolorize indigo blue, xylidine and malachite green. Highest decolorization values were observed with the 17-days-postinoculation cultures, which showed a 68% of decolorization for xylidine after 120 min of contact time, while malachite green was degraded up to 90% after 90 min, both decolorization values were reached by using cultures based on soya bran, while cultures based on the mixture showed to be more efficient to degrade indigo blue (95% after 30 min). As it was revealed by PAGE, there was a correspondence of decolorization bands and laccase activity bands, thus, laccase activity could be associated with the process of dye decolorization. Detoxification assays were carried out by using the extraction liquid from malachite green degradation. Detoxification of malachite green was up to 100% after 2 h of contact time and using Phanerochaete chrysosporium as test organism.