Polyamines and proline are affected by cadmium in roots and nodules of soybean

BALESTRASSE KB; GALLEGO SM; BENAVIDES MP; TOMARO ML

PLANT AND SOIL

Springer Netherlands

Lugar: Holanda; Año: 2005 vol. 270 p. 343 - 353

0032-079X

The effect of moderate (50 µM) and high (200 µM) doses of Cd were studied in relation to polyamine (Pas)metabolism, proline level and the glutamine synthetase/glutamate synthase system (GS/GOGAT) activity in nod-ulesand roots of soybean plants during 6 days of treatment. The lower Cd concentration increased putrescine (Put)in both nodules and roots, while 200 µM Cd increased Spm only in nodules and Put in roots. Spermidine (Spd)decreased in roots under both Cd concentrations. Arginine decarboxylase (ADC) and ornithine decarboxylase(ODC) were both involved in Put biosynthesis in roots. In nodules, Put formation could mainly be attributed toODC activity. Diamine oxidase (DAO) activity was severely reduced by 50 and 200 µM Cd either in nodules orroots. The GS/GOGAT system activity was depressed either with 50 or 200 µM Cd, but most significantly with thehighest metal concentration. Under 200 µM Cd, GS activity decayed to 25% or 60% of the control in nodules androots, respectively, while GOGAT decreased 85% in nodules and 79% in roots by day 4 of treatment. Ammoniumincreased greatly in nodules (200% over the controls) and roots (100%) under 200 µM Cd. Proline concentrationincreased significantly in nodules and roots under both Cd treatments, more markedly under 200 µM Cd. Therelationship between Pas and proline accumulation and nitrogen assimilation is discussed.