Phytochemical investigation and biological activities of Fusarium SP. An entomogenous fungus

MARCINKEVICIUS, KARENINA; SALVATORE, ANALIA; MURUA, GABRIELA; ARENA, MARIO; VERA, NANCY

Biocatalysis and Agricultural Biotechnology

Elsevier

Año: 2019 vol. 18

1878-8181

Entomopathogenic fungi have an incredible capacity to produce biologically active metabolites. The presentstudy was undertaken to evaluate the insecticidal activity of extracts, fractions and pure compounds fromFusarium sp. ARSEF 3300 entomopathogenic fungi against Spodoptera frugiperda Smith (Lepidoptera) and Ceratitiscapitata Wiedemann (Diptera). Additionally, antimicrobial activity was determined against Staphylococcus aureusATCC 6538 and Pseudomonas aeruginosa ATCC 27853. The culture media developed in the absence and presenceof remains of the S. frugiperda insect were called fungus (H) and fungus-insect (HI), respectively. Volatilecompounds in the extracts obtained with ethyl acetate were identified by GC-MS. Seven compounds of knownchemical structure were isolated: cholesterol (1), campesterol (2), palmitic acid (3), cis-Oleic acid (4), stearicacid (5), ester propyl myristate (6) and cis-9, cis-12 Linoleic acid (7). The ethyl acetate extract of the HI supernatantof Fusarium sp., showed the highest ingestion dissuading activity in S. frugiperda (83% at 300 μg/g ofdiet) and the highest oviposition deterrence in C. capitata (50% at 50 μg/cm2). Extracts of H and HI supernatantsfrom Fusarium sp. inhibited the growth of the P. aeruginosa (53.64% and 45.39%) and S. aureus (76.08% and79.61%) at 400 μg/mL. Palmitic acid (46.23% and 38.59%), cis-Oleic acid (49.95% and 42.33%) and stearic acid(50.44% and 39.72%) showed the highest inhibition of growth and biofilm production in S. aureus at 100 μg/mL.Our results suggest the possible utilization of entomopathogenic fungal metabolites in the control of insect pestsand human health