Purifcation of Recombinant Human Growth Hormone Using a Short Synthetic Peptide

S. L. SAAVEDRA; M.C. MARTÍNEZ-CERON; S. L. GIUDICESSI; G. FORNO; F. ALBERICIO; O. CASCONE; S. A. CAMPERI

Whistler

Simposio; American Peptide Symposium 2017; 2017

American Peptide Society

Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although there are several expression systems used for its production, the milk from transgenic cows is one of the most interesting due to the high levels of rhGH achieved (5 g/l). We have designed and synthesized a short peptide using Fmoc chemistry and studied its ability to purify rhGH from milk once immobilized on a Sepharose support. The adsorption isotherm for the rhGH-peptide system showed a dissociation constant of 3.1 × 10-6 M, more moderate than those of antibodies, which are in the order of 10-9 M. Once performed the chromatography between our generated peptide-resin and spiked milk with rhGH, we analyzed with SDS-PAGE the wash and elutionfractions and observed that rhGH was purified with over 90% purity in a single step. This proves that short peptides are an efficient and economical tool for the purification of therapeutic proteins from complex mixtures.