Combined effect of doping salts and matrices on the UV-MALDI-TOF-MS analysis results

ROSA ERRA-BALSELLS; SILVANA L. GIUDICESSI; FRANCO M. CABRERIZO; DAISUKE HIROTA; HIROSHI NONAMI

Osaka, Japón.

Congreso; 54th Annual Conference on mass Spectrometry; 2006

Mass Spectrometry Society of Japan

Salt impurities that may originate from the matrix, the analyte, solvents, glassware, surface of sample carrier, etc. may results in alkali metal adducts appearing in the UV-MALDI-MS. This is sometimes undesirable and can complicate de mass spectra. However, some species such as synthetic polymers and carbohydrates are primarily ionized by the formation of metal ion adducts (cationization). For this reason metal salts are often added to samples in order to enhance the analyte signals. Furthermore, cationization is somewhat matrix dependent. Understanding cationization mechanisms and the combined salt-matrix effects on it, are obviously required for better control of ion formation in UV-MALDI process.