IDENTIFICATION OF A FUNGAL STRAIN WITH CELLULOLYTIC CAPACITY, NATIVE OF THE PROVINCE OF MISIONES.

RODRIGUEZ, DANIELA; ALONSO PAIVA, IVAN; CASTRILLO, LORENA; ZAPATA, PEDRO DARÍO; VILLALBA, LAURA

Rosario

Congreso; L Congreso SAIB 2014; 2014

SAIB

Since several years, many species of white rot fungi with interesting characteristics for use in biotechnological applications are being described. Applications of cellulases from new microorganism in industrial and environmental technologies require large amounts of enzymes at low cost. So it is necessary to select and identify new organisms with elevated synthesis of these enzymes. The aim of this study was to identify a fungal strain with cellulolytic capacity belonging to Phylum Basidiomycota, with the help of molecular and bioinformatic tools. From morphological identification in the correct phyllum of a strain with cellulolytic capacity, DNA was extracted insufficient quantity and quality and ITS1-5,8S-ITS2 regions were amplified and sequenced using universal primers ITS 1 and ITS 4. To compare the obtained information with existing databases, the BLASTn (Basic Local AlignmentSearch Tool) tool of NCBI (National Center for Biotechnology Information) was applied, and contig consensus with the CAP 3 program was performed. 39 sequences were selected to be analyzed phylogenetically with the MEGA 6program using Maximum Parsimony, Maximum Likelihood and Neighbor-Joining methods. Consequently, by constructing monophyletic clades, it was possible to identify the fungal strain with cellulolytic capacity, native ofMisiones as Pycnoporus coccineus with a very high bootstrap value (>90%).