Triolein reduces MMP-1 upregulation in dermal fibroblasts generated by ROS production in UVB-irradiated keratinocytes

LEIROS GJ; KUSINSKY, AG; BALAÑA , ME; HAGELIN K

JOURNAL OF DERMATOLOGICAL SCIENCE

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2017 p. 124 - 130

0923-1811

Background: Cytokine production and oxidative stress generated byultraviolet radiation B (UVB) skin exposure are main factors of skinphotoaging. Interleukin-6 (IL-6) produced by irradiated keratinocytes isproposed to have a role in metalloproteinases (MMPs) expressionactivation in dermal fibroblasts.Objectives: We examined the effect of triolein treatment of UVBirradiatedkeratinocytes on MMP1 (interstitial collagenase) expressionresponse of dermal fibroblasts. We assayed UVB-irradiated keratinocytessoluble signals, mainly IL-6 and reactive oxygen species (ROS).Methods: IL-6 expression and ROS generation were assayed in UVBirradiatedkeratinocytes. MMP1 mRNA expression response was assayed infibroblasts grown in keratinocytes conditioned medium. We evaluated theeffect of treating keratinocytes with triolein on IL-6 expression and ROSgeneration in keratinocytes, and MMP1 expression in fibroblasts.Results: The irradiation of epidermal cells with sublethal UVB dosesincreased IL-6 expression and ROS generation. Conditioned culture mediumcollected from keratinocytes was used to culture dermal fibroblasts. MMP1mRNA expression increase was observed in fibroblasts cultured in mediumcollected from UVB-irradiated keratinocytes. Triolein treatment reducedthe IL-6 expression and ROS generation in keratinocytes and this effectwas reflected in downregulation of MMP1 expression in fibroblasts.Conclusions: Triolein reduces both the expression of IL-6 and ROSgeneration in irradiated keratinocytes. It seems to exert an antiinflammatoryand anti-oxidative stress effect on irradiated keratinocytesthat in turn reduces MMP1 expression in dermal fibroblasts.Collectively, these results indicate that triolein could act as aphotoprotective agent.