INVESTIGADORES
BALAÑA maria eugenia
congresos y reuniones científicas
Título:
ANDROGENS ALTER DERMAL PAPILLA SECRETED FACTORS INVOLVED IN HAIR FOLLICLE STEM CELL DIFFERENTIATION
Autor/es:
LEIRÓS, GUSTAVO; DEL PRIORE, SABRINA; BALAÑÁ, MARÍA EUGENIA
Lugar:
Tucumán
Reunión:
Congreso; 45th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2009
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Hair follicle (HF) formation begins when signals from the
mesenchyme-derived dermal papilla (DP) reach multipotent
epidermal stem cells in the bulge region. The growth of human HF
from beard, axillas and scalp is susceptible to androgen action by
modulation of DP activity. In androgenetic alopecia (AGA)
androgen action causes HF miniaturization and baldness through a
mechanism(s) which remain unclear.
Circulating androgens act on DP cells probably by altering the
regulatory paracrine factors involved in the differentiation and
proliferation of the HF multipotent cells. Bidimensional gels
performed with proteins from conditioned culture media of DP cells
revealed differences in the profile of DP secreted protein in response
to androgen action. In order to evaluate the role of epithelialmesenchymal
interactions in HF cell differentiation, we established
a co-culture model with DP cells from human biopsies of patients
suffering AGA and HF stem cell line (Tel E6/E7). The expression
levels of K6hf, keratin used as hair differentiation marker, were
lower in Tel E6/E7 co-cultured with androgen-treated DP cells, both
by western blot and real time PCR.
We conclude that androgens, modulating the DP secretory activity,
would alter the expression of specific genes involved in normal HF
stem cell differentiation, de-regulating hair follicle regeneration and
inducing miniaturization.