TNF-alpha promoter SNPs and HPV cervical infection in guarani indian women of Misiones, Argentina.

BADANO I; QUINTERO I; CABRERA M; TONON SA; CAMPOS R; PICCONI MA; LIOTTA DJ

Beijing, China.

Conferencia; 24th International Papillomavirus Conference and Clinical Workshop.; 2007

International Papillomavirus Society

The TNF-alpha has been implicated in the control of HPV infection by induction of apoptosis in HPV-infected cells and cervical cancer cells and stimulation of the inflammatory response. Single Nucleotide Polymorphisms (SNPs) located in the promoter region of the gene (positions -237; -307; -375; -863; -857) have been reported to influence TNF transcription, and therefore may play a role in the pathogenesis of cervical cancer, but results are contradictory. Objective: We investigated the potential association between SNPs positions -237, -307 and High Risk HPV (HR-HPV) infection in Native American women of the Guaraní-Mbya population settled in Misiones. We study 60 samples of total genome from cervical cells (21 HPV negative and 39 HPV-HR positive), which epidemiological and HPV typing data were previously published Tonon et al., Int J Infect Dis, 2003). TNF alpha SNPs were analyzed by PCR and sequencing. Potential association between TNF genotypes and HPV infection were calculated by Odds Ratio. Resultados: The distribution of genotypes for the SNPs -237 was 93% (56/60)homozygous GG and 7% (4/60) heterozygous AG. The OR analysis showed no statistical significant association between genotype AG and HR-HPV infection O.R. 0.5 (IC95% 0.07-3.9). For the SNPs-307, we found only the common G allele in the population, so potential association effects could not be assessed. Conclusiones: Some authors have suggested a protective effect of SNP -237A against cancer; in our work the frequency of allele A was lower in HR-HPV infected women compared with negative (O.R. 0.5 [IC95% 0.07-3.9]), but not statistically significant. In the other hand the risk allele 307A was not found in this population. Although the small sample size, this work provides initial data to extend our analysis to a regional case control study and further understand the role of ethnic and geographical factors in the development of disease.Supported by the Int. Soc. for Infect.Dis. and Agencia Nacional de Promoción Científica y Técnica (PICT R 311/03), Argentina.