INVESTIGADORES
AYUDE Maria alejandra
artículos
Título:
BACILLUS SUBTILIS AS A BIOINDICATOR FOR ESTIMATING PENTACHLOROPHENOL TOXICITY AND CONCENTRATION
Autor/es:
M. ALEJANDRA AYUDE; ELENA OKADA; J. FROILAN GONZALEZ; PATRICIA M. HAURE; S. MURIALDO
Revista:
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Editorial:
Springer
Referencias:
Año: 2009 vol. 36 p. 765 - 768
ISSN:
1367-5435
Resumen:
t Pentachlorophenol (PCP) and its sodium salt
(Na-PCP) are extremely toxic chemicals responsible for
important soil and groundwater pollution, mainly caused
by wastes from wood-treatment plants, because chlorinated
phenols are widely used as wood preservatives. The
methods most commonly used for routine analysis of
pesticides such as PCP and Na-PCP are high-performance
liquid chromatography (HPLC) and gas chromatography?
mass spectroscopy (GC?MS). A variety of rapid biological
screening tests using marine organisms, bioluminescent
bacteria, and enzymes have also been reported. In this
study, rapid biological screening analysis using Bacillus
subtilis was developed, to assess the biodegradation of PCP
and its by-products in liquid samples. An empirical model
is proposed for spectrophotometric analysis of Na-PCP
concentration after growth of Bacillus subtilis.