LACCASE AND LIGNIN PEROXIDASE ACTIVITY IN INDIGENOUS Pleurotus sp. AND Trametes sp. STRAINS

CARRILLO ANGELES; JOSÉ BONILLA; KURINA-SANZ, MARCELA; AGUIRRE PRANZONI CB

MENDOZA

Congreso; XL REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2022

SBC

The advances in biotechnological processes to obtain high value products are intimately related to enzyme production. The use of enzymes substantially improves the sustainability of industrial processes. Specifically, the lignin-modifying enzymes (LMEs) are attracting the attention because they allow reducing lignocellulosic waste and can biodegrade recalcitrant products. The white rot fungi are known as promising laccase and peroxidase producers. The main objective of this work is to explore the enzyme producing capacity of two white rot fungi: Pleurotus sp. and Trametes sp., in the presence of lignocellulosic wastes, using both liquid and solid fermentations. The fungal strains were isolated from a rural zone of San Luis, Argentina. Brewer's bagasse from a local brewing industry was used as lignocellulosic substrate for solid fermentation or it was also added to YG liquid medium. The laccase and lignin peroxidase activities were determined using ABTS and pirogalol, respectively. When YG media were supplemented with brewer's bagasse, the laccase activity increased from 320.56 to 624.64 U/L in Pleurotus sp. and from 3.20 to 104.46 U/L in Trametes sp. On the contrary, lignin peroxidase activity did not increase in the presence of lignocellulosic substrate neither for Pleurotus sp. nor for Trametes sp. Solid fermentation analysis yielded promising results in terms of lignin peroxidase activation, since both microorganisms increased twenty-fold the lignin peroxidase activity (0.0 to 20.0 U/hL), when compared to liquid supplemented culture. Under the same conditions, Trametes sp. triplicated the laccase activity (104.46 to 365.55 U/L), while Pleurotus sp. halved it (624.64 to 378.24 U/L). These results show that culture conditions determine the differential enzyme production, which can be optimized by using lignocellulosic waste as enzyme activity-inducing substrate.