The distribution of mannose-6-phosphate receptors changes from newborns to adults in rat liver

GUILLERMO JOFRÉ; CARVELLI LORENA; AGUILERA CAROLINA; TIRSO SARTOR; ADRIANA GONALEZ-JATUFF; SOSA ESCUDERO MIGUEL

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2011 p. 208 - 211

0006-291X

The co-existence of two types of mannose-6-phosphate receptors (CD-MPR and CIMPR)in most cell types is still not well explained. Some evidence suggests that the CIMPRcould be actively involved in the regulation of growth factors in the early stages ofmammalian organ development. In this study, it was demonstrated that both receptorsare distributed in a non-overlapping fashion in rat liver, and that the distribution of CIMPRchanges over a percoll gradient between newborn and adult animals. By usingmarker proteins it was observed that in newborns the CI-MPR is located both inintracellular fractions and in fractions that coincide with a plasma membrane marker,whereas in adults it is only detected in intracellular fractions. It was also noted that Nacetyl-b-D-glucosaminidase distribution is closer to CI-MPR than to CD-MPR and thatacid phosphatase did not match with any receptor. This evidence may also suggest thatboth receptors have different functions, mainly at early stages in the development oforgans.