Efectos de la deficiencia de vitamin A sobre los procesos de apoptosis y proliferación celular de la glándula mamaria de rata.

VAZQUEZ, MIRIAM E; FERRARI C; FILIPPA, VERÓNICA; ACOSTA, MARIANO; MOHAMED, FABIAN; GIMENEZ, MARÍA SOFÍA

San Luis

Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biología de Cuyo

The mammary gland development is a complex program of cell proliferation and differentiation. We study if vitamin A deficiency alters the expression of apoptotic and proliferative proteins in mammary gland. Female Wistar rats at weaning were divided into six groups. One group was fed with a vitamin A-sufficient diet (A), another with a vitamin A-deficient diet (-A) for 3 and 6 months, and the third group with -A diet for 75 and 150 days, respectively, and after which they received A diet for 15 and 30 days, respectively. BAX, BCL-2 and PCNA proteins were detected by immunohistochemistry and apoptotic cells by TUNEL assay, and BAX and BCL2 mRNA by RT-PCR. In (-A) group, the number of apoptotic nuclei in glandular cells increased in relation to control groups (3 and 6 months). In (-A) group, the immunostaining of BAX, PCNA and BCL-2, and the expression of BAX and BCL-2 mRNA were increased in relation to the control group. Vitamin A deficiency induces apoptosis in mammary gland after 3 and 6 months. The proliferation marker (PCNA) increases in (-A) group and was reverted with the vitamin A-sufficient diet. Vitamin A deficiency provokes changes in apoptotic and cell proliferation processes of rat mammary gland.