EFFECTS OF A HIGH FAT DIET ON TEMPORAL PATTERNS OF METABOLIC PARAMETERS

ALFONSO JO; LOPEZ M; NAVIGATORE FONZO LS; ANZULOVICH, AC

TAFI DEL VALLE, TUCUMAN

Congreso; XXXV ANNUAL SCIENTIFIC MEETING; 2018

ASOCIACION DE BIOLOGIA DE TUCUMAN

The worldwide prevalence of obesity has increased dramatically during the last decades. The etiology of obesity is multifactorial, and includes genetic, environmental and dietary factors, where hypercaloric diets play a central role in the development of the disease. Recent studies have linked metabolic homeostasis with the circadian clock at the molecular, physiological and behavioral levels. The objective of this work was to investigate the effects of a high saturated fat diet on daily patterns of metabolic parameters in Holtzman rats. The animals weaned at 21d of age were randomly separated and fed with a normocaloric diet (ND group) and a high saturated fat diet (HFD group) for 12 weeks. Rats were maintained under 12h-light:12h-dark conditions during the treatment period. On the experiment day, they were sacrificed every 6hs and blood samples were collected. Serum glucose, triglycerides, total cholesterol, HDLc and LDLc+VLDLc were determined by colorimetric assays. We found that glucose and triglyceride levels did not vary throughout the day in any of the experimental groups. However, HDLc and LDLc+VLDLc cholesterol levelsoscillated in antiphase in the serum of the ND rats over a 24h period. Twelve weeks of HFD feeding increased circulating total cholesterol and cholesterol associated with the LDL-VLDL lipoprotein fraction at the beginning ofthe light period, while HDLc levels decreased during the dark phase. Thus, we can conclude that a diet based on saturated fats modifies the daily patterns of lipid parameters, increasing risk-associated LDLc+VLDLc levels at thebeginning of the rest period and decreasing safe-related HDLc levels during the activity period in rats.