Do You Want A Drink? Cardiovascular Oxidative Stress Produced By Drinking Cd Contaminated Water.

FERRAMOLA, M.; CORIA, M; BIAGGIO, V.; ANZULOVICH A.C.; GIMÉNEZ MS

Mar del Plata, Bs. As.

Congreso; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2007

SAIB

Cadmium intoxication increases oxidative stress parameters inseveral tissues. Objectives: The aim of this study was to assess thecardiovascular effect of drinking cadmium contaminated water.Experimental model: Male Wistar rats were separated into fourgroups: 1 (Controls, tap water), 2, 3 and 4 (tap water with 15 ppmCd ion, during 15, 30 and 60 days respectively). Food and waterwere administrated ad libitum. Afterwards, animals weredecapitated, under light ether anesthesia. Samples were kept at -20ºC, until processed. Methods: Determinations in serum:TBARS(Jentzsch ., 1969), Proteins (Layne ., 1957), NO (Schulkz., 1999), and Paraoxonase-1 (PON-1) (Beltowsky ., 2002).TBARS and iNOS expression (Western Blot) was assessed inheart. Results: Serum: TBARS were elevated in groups 3 and 4,(p<0.05). PON-1 activity in group 3 was the highest (p<0.001).PON-1 activity augmented in group 4 compared to controls(p<0.001). NO determination showed no differences among thegroups. Heart:TBARSincreased in group 4, (p<0.05). It was seen adecrease in iNOS production in groups 2 and 3. Conclusions: Ratsexposure to oral cadmium intoxication increases oxidative stressparameters in serum and heart. It also seems to be an induction ofantioxidant enzymes (PON-1) in serum at the first stages ofintoxication, with a decreased iNOS concentration in heart.