INVESTIGADORES
ANZULOVICH MIRANDA Ana cecilia
congresos y reuniones científicas
Título:
CLOCK’S DIFFERENTIAL TRANSCRIPTIONAL CONTROL ON OGG1 AND APE1 CIRCADIAN EXPRESSION
Autor/es:
CASTRO-PASCUAL, I; DAS NEVES OLIVEIRA, A; MELENDEZ, M; CARGNELLUTTI E; ALTAMIRANO FG; FERRAMOLA, M.; LACOSTE MG; DELGADO SM; ANZULOVICH AC
Lugar:
MENDOZA
Reunión:
Congreso; XL REUNION CIENTIFICA DE LA SOCIEDAD DE BIOLOGIA DE CUYO; 2022
Resumen:
The circadian clock integrates external environmental changes with the internal physiology. Different studies havedescribed a role of clock molecular machinery on the regulation of DNA repair mechanisms. In this sense, otherauthors reported a clock controlled modulation of DNA nucleotide excision repair. Accordingly, we previouslyreported evidence of circadian rhythmicity in the expression of genes involved in DNA base excision repair (BER)system, in 22-mo-old rats. BER is a key mechanism to avoid oxidative and alkylative DNA damage, whichpredisposes to different diseases such as cancer or neurodegenerative disorders. Our objective was to elucidatethe molecular mechanisms involved in the control of the circadian expression of the enzymes involved in the BERsystem. Through in vitro transient transfection studies in NIH-3T3 cells, we assayed the response of the regulatoryregions of Ogg1 and Ape1 genes to de BMAL1:CLOCK heterodimer. Previously, our bioinformatics studiesrevealed 13 E-box-like (CANNTG) and 5 perfect (CACGTG) E-box sites in regulatory regions of Ogg1 and Ape1,respectively. Subsequently, the bioluminescence assays showed that the BMAL1:CLOCK heterodimer exerted adifferential regulation, activating the Luc expression driven by the regulatory region of Ogg1 (p