Aging modifies the oxidative status and the circadian expression of BDNF and its TrkB receptor in the hippocampus

ALTAMIRANO FG; DELLA VEDOVA MC; RINALDI TOSI, ME; DELGADO SM; ANZULOVICH AC; GOMEZ MEJIBA SE; LACOSTE MG

MAR DEL PLATA

Congreso; XXX CONGRESO DE LA SAN; 2015

SOCIEDAD ARGENTINA DE NEUROCIENCIAS

Hippocampus plays a key role in memory and learning and is especially susceptible to oxidative stress. We previously observed BDNF and TrkB mRNA as well as antioxidant enzymes rhythms are modified in the hippocampus of aged rats. We hypothesize that aging, thorough the modification of the oxidative pattern, can alter 24h-rhythmicity. To test that, we measured the levels of BDNF and TrkB proteins, lipid peroxidation (LPO), protein carbonylation (PC) and total antioxidant capacity (TAC) throughout a 24h cycle in the hippocampus of young and aged rats. Holtzman 3- and 22-months old rats were maintained under constant darkness conditions during 15 days before the experiment. BDNF and TrkB proteins as well as TBARS (LPO), PC and TAC were determined in samples isolated at 4h-intervals. BDNF and TrkB levels varied (P