Unravelling the Transcriptomic Response of Pleurotus Pulmonarius Lbm 105 to Oxidative Stress in the Presence of Polychlorinated Biphenyls

CHELALICHE ANIBAL SEBASTIAN; ALVAREGNA ADRIANA ELIZABET; BENITEZ SILVANA FLORENCIA; ZAPATA PEDRO DARIO; FONSECA MARÍA ISABEL

Social Science Research Network

Elsevier

Año: 2023

Polychlorinated biphenyls (PCBs) are known persistent organic pollutants. Currently, they are considered one of the top priority hazardous substances by the U.S. Environmental Protection Agency´s superfund program. The white-rot fungus Pleurotus pulmonarius LBM 105 is capable of achieving high rates of removal of these compounds. A comparative transcriptomic study of this strain was carried out in the presence of Aroclors 1242, 1254 and 1260 to understand the underlying metabolism. A total of 231 differentially expressed genes (DEGs) were identified in the presence of a high concentration of the Aroclor mixture. Four principal metabolic categories were affected by the presence of PCBs. Oxidative metabolism exhibited the highest number of DEGs, with an increase in the expression of cytochrome P450 and cupredoxin-domain containing proteins. All protein-coding genes involved in xenobiotic metabolism showed elevated expression, including a significant representation of dienelactone hydrolases. The expression profile involving lipid metabolism suggests a transcriptional response to lipid peroxidation due to oxidative stress. Carbohydrate metabolism showed a notable decrease in its regulation. Thus, the metabolism of P. pulmonarius LBM 105 adapts to the removal of PCBs by modulating the transcription of enzymes necessary for their modification and by regulating the resistance to oxidative stress and lipid peroxidation.