Cell division visualized: barley reporter lines for chromosome and microtubule dynamics

KATERINA KADUCHOVÁ; CINTIA MARCHETTI; MIROSLAV OVECKA; PETR GALUSZKA; VÉRONIQUE BERGOUGNOUX; JOZEF SAMAJ; ALES PECINKA

PLANT JOURNAL

WILEY-BLACKWELL PUBLISHING, INC

Año: 2023 vol. 115 p. 600 - 601

0960-7412

Mitosis and cytokinesis are fundamental processes through which somatic cells increase their numbers and allow plant growth and development. Here, we analyzed the organization and dynamics of mitotic chromosomes, nucleoli, and microtubules in living cells of barley root primary meristems using a series of newly developed stable fluorescent protein translational fusion lines and time-lapse confocal microscopy. The median duration of mitosis from prophase until the end of telophase was 65.2 and 78.2 min until the end of cytokinesis. We showed that barley chromosomes frequently start condensation before mitotic pre-prophase as defined by the organization of microtubules and maintain it even after entering into the new interphase. Furthermore, we found that the process of chromosome condensation does not finish at metaphase, but gradually continues until the end of mitosis. In summary, our study features resources for in vivo analysis of barley nuclei and chromosomes and their dynamics during mitotic cell cycle.