Analysis of Laurdan fluorescence spectra in vesicles formed by ester and ether lipids

GONZÁLEZ PAZ AGUSTÍN NICOLÁS; GIMÉNEZ RODRIGO; FRÍAS MARÍA DE LOS ÁNGELES; DISALVO ANIBAL

San Luis

Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Biofísica; 2019

Universidad Nacional de San Luis

Many works study the behaviour of model membranes of varied characteristics using fluorescent probes sensitive to their environment. Among them Laurdan is specifically sensitive to the hydration of the membrane interphase.1 The membrane interphase has dynamic and dielectric properties different from those of the bulk aqueous phase and the hydrophobic region. With this purpose, the Generalized Polarization (GP) is calculated from the intensity of the emission and excitation wavelengths.1In this work, we report that using different emission maxima in gel phase and liquid crystalline phase different curves for GP of Laurdan vs temperature can be built.