Black Bean growth promotion and protection against seedborne pathogens by Trichoderma spp. from Northwest Argentina

DARFE, CAMILA; ZURITA, ALICIA; VILLAGRA, JORGE; PALACIOS, GUILLERMO; MERCADO, GUADALUPE; HARRIES, ELEONORA

Congreso; 16th International Trichoderma & Gliocadium Workshop; 2020

Cancelado por COVID Black Bean growth promotion and protection against seedborne pathogens by Trichoderma spp. from Northwest ArgentinaPreviously, we have isolated Trichoderma spp. strains from soils of Northwest Argentina (NWA) and characterized by antagonistic properties against Rhizoctonia solani (Rs.). In this study, was analysed the Trichoderma spp. application onto Black Bean for growth promotion and protection. Conidial suspensions of eight Trichoderma spp. strains (T1, T2, T3, T4, T5, T6, T7, T8) were inoculated on beans in two experimental approaches: blotter test and bioassays on pots under environment controlled conditions. After 7-10 days post-inoculation (dpi) in the blotter test, the seed germination and pathology were checked. High percentages of bean seed germination were registered in all treatments with exception of T5. The development of different natural seedborne pathogens as Rhizopus spp., Penicillium spp., Aspergillus spp., Fusarium spp., Rhizoctonia spp. and bacteria was observed. Interestingly, T1, T3 and T8 were able to reduce the seed colonization by pathogens. Mycoparasitism of these strains on Rhizoctonia spp. hypha were confirmed by microscopic analysis. In the bioassays, different growth parameters on bean plants as diameter of basal stem, number of trifoliate leaf, height and fresh weight were measured at 15 dpi. Significantly, T3, T1, T4 increased the plant height compared with the control without Trichoderma spp. Trifoliate leaves number, diameter of basal stem and fresh weight of bean plants inoculated with T8 and T3 were higher than the control. Also, these strains increased the root length and weight. Dates shown here allow concluding that T8 and T3 were the most effective to biocontrol seedborne pathogens and promote the black bean growth. Financial support: PRESTAMO BID PICT2016 N°4431, CIUNSA N° 2574