PHOSPHOLIPASE D 1 AND 2 INHIBITION PREVENTS OXIDATIVE STRESS IN RETINAL PIGMENT EPITHELIUM CELLS EXPOSED TO HIGH GLUCOSE LEVELS

ECHEVARRIA M. S.; TENCONI P. E.; MATEOS M.

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

Oxidative stress (OE) and inflammation are involved in the pathogenesis of several retinal diseases. We previously demonstrated that classical hospholipase D isoforms (PLD1 and 2) mediate the inflammatory esponse of retinal pigment epithelium (RPE) cells induced by high glucose (HG) levels. Furthermore, a significant increase in reactive oxygen species (ROS) was observed in RPE cells exposed to HG. The aim of the present work was to study the relationship between OE and PLD activation observed in HG- treated RPE cells. Methods: RPE cells (ARPE-19) were exposed to HG (33 mM) or to normal glucose levels (NG, 5.5 mM) for 24 h. To inhibit PLD1, PLD2 and cyclooxygenase-2 (COX-2) VU0359595 (PLD1i, 0.5 μM), VU0285655-1 (PLD2i, 0.5 μM) or celecoxib (10 μM) were used, respectively. ROS production was assessed using the probe DCDCDHF. Immunocytochemistry assays (ICC) and western blots were performed to evaluate nuclear factor erythroid 2?related factor2 (Nrf-2) pathway. Results: HG-exposure for 24 h increased ROS levels (148%) in ARPE-19 cells with respect to NG. The incubation with PLD1i and PLD2i prevented HG-induced ROS generation in RPE cells. On the contrary, the inhibition of COX-2 was not able to prevent OE induced by HG. ICC showed Nrf-2 nuclear translocation in cells exposed to HG and this effect was not observed when cells were pre-treated with PLD1i and PLD2i. Nrf-2 activation correlated with and increased heme oxygenase-1 (HO-1) expression (42%) in HG-exposed cells but no differences were observed in cells treated with PLD1i or PLD2i with respect to NG. Conclusions: Our results demonstrate that PLD1 and PLD2 inhibition not only prevents the inflammatory response of RPE cells, but also decreases OE generated in RPE cells exposed to HG in a Nrf-2 and COX-2 independent manner. Further experiments are needed to fully elucidate the mechanisms by which the PLD pathway mediates OE in RPE cells exposed to inflammatory injury.