IMPACT OF THE AZITHROMYCIN ADMINISTRATION ON INTESTINE IMMUNE CELLS: FOCUS ON SEX AND MOUSE STRAIN

PIQUERAS, VIRGINIA ALEJANDRA;; CORREA, SILVIA GRACIELA

Congreso; Reunión de sociedades de biociencias 2020; 2020

Azithromycin (AZM) is a widely used antibiotic, with additional antiviral and anti-inflammatory properties not well understood yet. It belongs to the family of macrolide antibiotics and is approved worldwide to treat community-acquired infections. AZM is widely distributed in tissues and body fluids. Although short-term treatment results in decrease of richness, diversity and taxonomic composition of the gut microbiota, long-term effects are unknown. The impact of acute administration of AZM in drinking water (50 mg/kg/day/5 days) was evaluated in lymphocyte (L) subsets and cytokine microenvironment of mesenteric lymph nodes draining small intestine (SI) and colon (C) of 3 mouse strains: C57BL76, Per2KO (clock gene KO) and Foxp3-GFP. Using flow cytometry, we determined frequency (%) and absolute number (#) of CD3+L and CD19+L in adult male and female hosts. In C56BL6 males, no differences were observed between control and treated groups. In Per2KO males the % of CD3+L decreased in SI and C (p