Dual effects of AMP-Activated Protein Kinase (AMPK) in Isolated Rat Atria Subjected to Simulated Ischemia-Reperfusion Depending on the Energy Substrates Available

HERMANN, ROMINA; MESTRE CORDERO, VICTORIA E.; FERNÁNDEZ PAZOS, M.MERCEDES; REZNIK, FEDERICO; VÉLEZ, DÉBORA E.; SAVINO, ENRIQUE A.; MARINA PRENDES, M.GABRIELA; VARELA, ALICIA

Buenos Aires

Congreso; III International Congress in Translational Medicine; 2016

AMPK functions as a cellular fuel gauge, being activated when cellular energy is depleted to restore homeostasis at a cell-autonomous level. Whether AMPK plays a critical role in myocardial ischemia-reperfusion remains uncertain. In this study, we explored the effects of the pharmacological inhibition of AMPK by Compound C (CC;10 μM) in isolated rat left atria subjected to 75min Is-75min Rs after 75 min stabilization period. Since levels of fatty acids are elevated in most clinical situations of ischemia-reperfusion, the influence of palmitate (P) was also studied. For this aim, atria were incubated in Krebs?Ringer containing 10mM glucose or 10mM glucose and 1,2mM P as exogenous substrates, 95% O2?5% CO2, pH 7,4. For Is, the incubation medium contained 10mM 2-deoxy-D-glucose, 95% N2?5% CO2, pH 6,8. ANOVA, n=8.Results showed a significant increase in AMPK endogenous activation during Is, remaining activated during Rs. CC prevented it during the whole experiment. During Is, peak contracture developed earlier in the atria stabilized with P (20-min vs 40-min Is, p