BECAS
FERNÁNDEZ PAZOS MarÍa De Las Mercedes
artículos
Título:
Differential effects of AMP-activated protein kinase in isolated rat atria subjected to simulated ischemia-reperfusion depending on the energetic substrates available.
Autor/es:
ROMINA HERMANN; VICTORIA EVANGELINA MESTRE CORDERO; MARÍA DE LAS MERCEDES FERNÁNDEZ PAZOS; FEDERICO JOAQUÍN REZNIK; DÉBORA ELISABET VELEZ; ENRIQUE ALBERTO SAVINO; MARÍA GABRIELA MARINA PRENDES; ALICIA VARELA
Revista:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2017
ISSN:
0031-6768
Resumen:
AMP-activated protein kinase (AMPK) is a serine-threonine kinase that functionsprimarily as a metabolic sensor to coordinate anabolic and catabolic processes in thecell, via phosphorylation of multiple proteins involved in metabolic pathways, aimed toreestablish energy homeostasis at a cell-autonomous level. Myocardial ischemia andreperfusion represents a metabolic stress situation for myocytes. Whether AMPK playsa critical role in the metabolic and functional responses involved in these conditionsremains uncertain.In this study, in order to gain a deeper insight into the role of endogenous AMPKactivation during myocardial ischemia and reperfusion, we explored the effects of thepharmacological inhibition of AMPK on contractile function rat, contractile reserve,tissue lactate production, tissue ATP content and cellular viability. For this aim, isolatedatria subjected to simulated 75 min ischemia-75 min reperfusion (Is-Rs) in thepresence or absence of the pharmacological inhibitor of AMPK (Compound C) wereused. Since in most clinical situations of ischemia-reperfusion the heart is exposed tohigh levels of fatty acids, the influence of palmitate present in the incubation mediumwas also investigated.The present results suggest that AMPK activity significantly increases during Is,remaining activated during Rs. The results support that intrinsic activation of AMPK hasfunctional protective effects in the reperfused atria when glucose is the only availableenergetic substrate whereas it is deleterious when palmitate is also available. Cellularviability was not affected by either of these conditions.
