LOADING OF CANNABIS SATIVA OIL EXTRACT ON HYDROGELS AND 3D PRINTED SCAFFOLDS

ANTEZANA PABLO EDMUNDO; MUNICOY , SOFIA; DESIMONE MARTIN F

Mar del Plata

Congreso; Proceedings of the XII Latin-American Congress of Artificial Organs and Biomaterials; 2023

Introduction and objective: Nowadays, biomaterials with therapeutic molecules play an active role in wound healing and infection prevention. 3D printing approach has emerged to overcome several of the major deficiencies of tissue engineering (1). Cannabis sativa oil is known for its anti-inflammatory and analgesic effects and antioxidant activity (2). The aim of this work was the development of hydrogels loaded with cannabis sativa oil using different approaches.Methodology: We synthetized the collagen hydrogels and loaded with silver nanoparticles previously synthetized. Then the Cannabis sativa oil extract was added. For the characterization we performed transmission and scanning electron microscopy, fourier transform infrared, silver absorption and release, rheological properties, enzymatic Degradation of Collagen Gels, antioxidant capacity, antimicrobial activity and biocompatibility. On the other hand, to develop 3D scaffolds, we first synthesized the gelatin and alginate bioink. We set the bioprinting parameters and after the impression we lyophilized the scaffolds and loaded them with Cannabis sativa oil extract. Finally, we performed the same characterization previously described for the collagen hydrogels.Results and discussion: We were able to develop collagen hydrogels loaded with silver nanoparticles and Cannabis sativa oil extract. The presence of the silver nanoparticles gives interesting feature to the biomaterial such as improved mechanical properties, resistance to collagenase degradation and long-lasting antimicrobial effect. The antioxidant activity of Cannabis sativa oil successfully improved the biocompatibility and also enhances the antimicrobial activity against Gram positive and Gram negative bacteria during seven days in liquid medium of the nanocomposite. On the other hand, we developed a bioink made with gelatin and alginate that was able to be printed using an extrusion 3D bioprinter. The 3D scaffolds obtained were able to be lyophilized and when the elastic modes were assessed they show hydrogels properties. The swelling capacity of the 3D scaffolds was almost 800%. In this sense, the scaffolds were loaded with Cannabis sativa oil extract and the presence of the extract provided antimicrobial against Gram positive and Gram negative bacteria in both liquid and solid medium, and antioxidant activity to the 3D scaffolds.Conclusions: Altogether, these results suggest that collagen hydrogels loaded with silver nanoparticles and Cannabis sativa oil extract are a promising alternative to common treatments of wound infections and wound healing. In addition, the new biocompatible material printed with 3D technology and with the addition of Cannabis sativa oil could become an attractive alternative to common treatments of soft-tissue infections and wound repair.References1-Antezana, P. E., Municoy, S., Álvarez-Echazú, M. I., Santo-Orihuela, P. L., Catalano, P. N., Al-Tel, T. H., ... & Desimone, M. F. (2022). The 3D bioprinted scaffolds for wound healing. Pharmaceutics, 14(2), 464.2-Wang, L.-L.; Zhao, R.; Li, J.-Y.; Li, S.-S.; Liu, M.; Wang, M.; Zhang, M.-Z.; Dong, W.-W.; Jiang, S.-K.; Zhang, M.; et al. Pharmacological activation of cannabinoid 2 receptor attenuates inflammation, fibrogenesis, and promotes re-epithelialization during skin wound healing. Eur. J. Pharmacol. 2016, 786, 128–136.