Use of blueberry waste to obtain bioactive compounds

DE LILLO MARIA FLORENCIA; HABIB NATALIA CECILIA; LIZARRAGA EMILIO

San Miguel de Tucuman

Jornada; XXXVI Jornadas Científicas de la Asociación de Biología de Tucumán; 2019

Asociación de Biología de Tucumán

Agroindustrial wastes represent significant amounts andhigh disposal costs transform them into an environmental and economic problem. In previous studies, we demonstrated that extracts of Vaccinium myrtillus L. (blueberry) stem present secondary metabolites with promising biological activities. The aim of this work was to obtain subextracts from a polar extract of blueberry stems and determine the chemical composition, antioxidant activity (AAO) and the possible antihyperglycemic effect. The acetonic extract (AE) of blueberry stems was partitioned with solvents of increasing polarity, obtaining hexane, methylene chloride, ethylacetate and hydroalcoholic fractions. Using the AE and the respective fractions, a chemical profile and the quantification of constituents was performed by spectrophotometric methods. The AAO was determined by scavenging of the ABTS+ and DPPH radicals; iron and molybdenum reduction and the inhibition of lipid peroxidation. The antihyperglycemic effect was evaluated in vitro by inhibition of α-glucosidase enzyme. The fraction ethyl acetate showed an important AAO probably due to the greater amount of phenolic compounds present (mainly flavonoids) respect to the other subextracts analyzed. In addition, thisfraction significantly (p≤0,05) inhibited the activity of the α-glucosidase enzyme in a dose-dependent manner. The partition of EA allowed to concentrate the metabolites according to their polarity and enhance both the antioxidant and antihyperglycemic activity. V. myrtillus L. stems represent a source of metabolites with promising bioactivity.