Allantoin modulates ROS production in Arabidopsis thaliana

BOGINO, MARÍA FLORENCIA; LESCANO, IGNACIO; DESIMONE, MARCELO; GONZÁLEZ, CLAUDIO ALEJANDRO

Córdoba

Congreso; XXXII Reunión Argentina de Fisiología Vegetal-XVI Congreso Latinoamericano de Fisiología Vegetal.; 2018

Sociedad Argentina de Fisiología Vegetal

In plants, several abiotic stresses lead to the overproduction of reactive oxygen species (ROS) which cause oxidative stress in cells. However, the functions of ROS have been largely discussed, being also implicated in the signaling of developmental processes and plant responses to stress.Allantoin, a purine-derived metabolite, is accumulated in plants exposed to different abiotic stresses and has been related with stress tolerance. The Ureide Permease 5 (AtUPS5) has been proposed as a mediator for its long distance transport. Interestingly, ups5 mutant seedlings were sensitive to salt suggesting that allantoin transport mediated by AtUPS5 is an important event for salt stress alleviation.To determine if AtUPS5-mediated allantoin long distance transport could be also involved in ROS levels regulation, WT plants and ups5 mutants were incubated with exogenous allantoin during 3 and 24 hours and after that ROS levels were determined. Allantoin content increased in WT shoots, but not in ups5 ones. After 3 hours of allantoin treatment, ups5 mutants decreased its O2?- levels compared to WT. However, 24 hours allantoin treatment led to a higher ROS production in the shoots of both genotypes. Our results suggest a role for allantoin transport in modulating ROS production.