SHORT ALCOHOL CONSUMPTION IMPAIRS THE OFFSPRING HEALTH IN MICE

GOTFRYD, LUCILA; STINSON, MARCELO GABRIEL; VERMEULEN M; ALCAIN J; VANZULLI S; CAMBIASSO, MAITE; NAGUILA, ZAIRA; ERLEJMAN, ALEJANDRA GISELLE; CALVO, JUAN CARLOS; SALOMONE, GABRIELA; FONTANA, VANINA ANDREA

Buenos Aires, Argentina

Congreso; IFPA 2019 - VIII SLIMP; 2019

IFPA & SLIMP

Previously, we observed that male alcohol consumption delayed embryo differentiation by deregulating peri-implantation events and altering embryo trophoblast and inner cell mass morphology in vitro. Objective: To evaluate the effects of paternal alcohol consumption on the offspring in mice. Methods: CF-1 male mice were exposed (treated group) or not (control group) to 15% (v/v) ethanol in drinking water ad libitum for 15 days. Control and treated males were mated with non-treated CF-1 females (1:1), and pregnant females (vaginal plug: day1) were determined. Pregnancy outcome was evaluated and litter mortality, weight and size registered. Blood from adult offspring was analyzed in a Coulter counter. Then, they were sacrificed and dissected. Offspring´s spleen was weighed and analyzed histologically. Spleen cell populations were determined by flow cytometry. Results: After 21 days of pregnancy, born mice from both groups were registered and, during the first week after birth, an increased number of deaths from treated group (p