Fe 3 O 4 nanoparticles mediated synthesis of novel spirooxindole-dihydropyrimidinone molecules as Hsp90 inhibitors

MADDELA, SRINUBABU; MAKULA, AJITHA; GALIGNIANA, MARIO D.; PARAMBI, DELLA G. T.; FEDERICCI, FERNANDO; MAZAIRA, GISELA; HENDAWY, OMNIA M.; DEV, SANAL; MATHEW, GITHA E.; MATHEW, BIJO

ARCHIV DER PHARMAZIE.

WILEY-V C H VERLAG GMBH

Año: 2019 vol. 352

0365-6233

Heat shock protein 90 (Hsp90) is a validated molecular chaperone considered as the new key recipient for cancer intervention. The current study illustrates the synthesis of novel spirooxindole-dihydropyrimidinones (4a?j) by Fe 3 O 4 nanoparticles intervened synthesis and their Hsp90 ATPase inhibitory activity was investigated by the malachite green assay. All the compounds in the study demonstrated a moderate to potent ATPase inhibitory profile, with IC 50 values ranging from 0.18 to 6.80 μM. Compounds 4j, 4h, 4f, and 4i exhibited maximum inhibitory potential with IC 50 values of 0.18, 0.20, 0.35, and 0.55 μM, respectively. They were found to be better than the standard drug, geldanamycin (Hsp9 ATPase inhibition IC 50 = 0.90 μM). Compounds 4h and 4j with IC 50 values of 22.82 ± 0.532, 20.78 ± 0.234 and 21.32 ± 0.765, 28.43 ± 0.653 µM showed significantly greater potencies against the MCF-7 and HepG2 cell lines, respectively. Compound 4j showed good antioxidant activities in the DPPH test and H 2 O 2 assay (IC 50 = 20.13.23 ± 0.32 and 23.27 ± 0.32 μg/mL) when compared with the standard ascorbic acid (IC 50 = 19.16 ± 0.20 and 20.66 ± 1.09 μg/mL). A molecular docking study was performed to observe the binding efficiency and steric interactions of the lead moiety.