Evaluation of the interaction between different liposome preparation and Trypanosoma cruzi developmental stages.

DIBLASI, LORENA; PORTAL, PATRICIO; PAVETO, CRISTINA; ROSI, PABLO

Puerto Madryn

Congreso; BIOCELL2010, Vol. 346 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2010

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

One of the major public health problems in Latin America is Chagas?disease which is caused by the kinetoplastid protozo an Trypanosoma cruzi. Transmission occurs mainly by triatominae insect vectors, but also by blood transfusion and congenital routes. Although successful programs in vector control and banked-blood screening have led to a decline in transmission, neither total eradication nor effective treatment of the disease has been achieved. The only current treatment relies on nifurtimox and benznidazole, both drugs show toxic side effects and limited efficacy. The main challenges in pharmacotherapy of Chagas?disease are to increase effectiveness of drug treatment and to prevent adverse effects. Nano-drug delivery systems (nanoDDS) represent useful means to selectively deliver the drug to intracellular targets. We assayed the interaction of different lipid composition and sizes of liposomes with epimastigotes, blood stream tripomastigote and amastigote form of T.cruzi. As phosphatidylcholine (PC) is widely used used and the main component of lipidic nanoparticles formulations, we prepared 100% egg PC, 100% soy bean PC and 50% egg PC-50% soy bean PC nnDDS. The source of PC, the nnDDS composition preparation as well as liposome size modifies the effects against parasitic cell. The main conclusion is that liposomes themselves are effective against T. cruzi cells.